Anti-IL-1β Antibody

This polyclonal antibody from Antibodies.com targets Interleukin-1 beta (IL-1β), a 17 kDa pro-inflammatory cytokine central to innate immunity and the regulation of fever, sepsis, and chronic inflammatory disease. Raised in rabbit and supplied as 100 µL of unconjugated affinity-purified reagent, the antibody is validated for Western blot, immunohistochemistry, and ELISA workflows across human, mouse, and rat samples.

It detects both the 31 kDa inactive precursor (pro-IL-1β) and the caspase-1-cleaved mature form, supporting studies of inflammasome activation, caspase-1 processing, and cytokine release in macrophage, monocyte, and microglia models. The reagent is widely used to investigate NLRP3 inflammasome signaling, IL-1β-driven arthritis, gout, inflammatory bowel disease, neuroinflammation, and tissue injury responses.

Cross-reactivity spans Human, Mouse, and Rat, enabling consistent antibody use across rodent and human specimen panels. For Western blot, IL-1β resolves around 17 kDa (mature) or 31 kDa (precursor); in IHC the antibody supports formalin-fixed paraffin-embedded sections, while ELISA applications pair the reagent in cytokine quantification protocols. Store the vial refrigerated; avoid repeated freeze-thaw cycles, and aliquot upon first use to preserve activity across long-running studies.