Anti-IKZF1 Antibody

The IKZF1 gene encodes Ikaros, a zinc-finger transcription factor that serves as a master regulator of lymphocyte specification and differentiation. Mutations in IKZF1 are linked to primary immunodeficiency syndromes and B-cell acute lymphoblastic leukemia, making this protein a long-standing focus of immunology and hematology research. The Antibodies.com rabbit polyclonal anti-IKZF1 antibody recognizes endogenous Ikaros across human, mouse, and rat samples, enabling investigators to study chromatin remodeling complexes and hematopoietic lineage commitment in cell culture models and tissue specimens.

Validated for western blot, immunohistochemistry, and immunofluorescence workflows, this unconjugated reagent supports detection of full-length Ikaros and dominant-negative short isoforms implicated in leukemogenesis. The 50 µl vial provides enough material for multiple blotting membranes and coverslip arrays, and aliquoting upon first use helps preserve titer across freeze-thaw cycles. Store at -20°C in working aliquots to minimize loss of activity, and avoid repeated freeze-thaw cycles.

The polyclonal format increases the likelihood of recognizing multiple IKZF1 epitopes, including regions outside the N-terminal DNA-binding zinc fingers commonly disrupted in mutant alleles. Researchers studying T-cell development, B-ALL pathogenesis, or chromatin regulation will find this reagent useful for mapping Ikaros localization in the nucleus and tracking isoform expression changes during differentiation. Each lot is affinity-purified from rabbit serum to reduce non-specific binding and to support cleaner signal in immunofluorescence and IHC.