Anti-IKZF1 Antibody

The IKZF1 gene encodes Ikaros (also known as LyF-1), a Krüppel-like zinc finger transcription factor that orchestrates lymphoid lineage commitment and hematopoietic stem cell fate decisions. This polyclonal reagent from Antibodies.com was raised in rabbit and is supplied as 100 µl of unconjugated antiserum suitable for western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples. Ikaros localizes predominantly to the nucleus where it remodels chromatin and recruits the NuRD-HDAC complex to silence or activate target genes involved in T-cell receptor rearrangement and B-cell maturation. Researchers studying acute lymphoblastic leukemia, common variable immunodeficiency, or hematopoiesis use antibodies like this one to probe IKZF1 expression across developmental stages and to track the loss of Ikaros activity in leukemic blasts where deletions in chromosome 7p are recurrent. The unconjugated format allows flexible pairing with custom fluorophores, biotin, or HRP depending on the assay, and the polyclonal nature broadens epitope coverage across the multiple zinc finger domains that define this transcription factor family. For best results, aliquot upon arrival and store at 4 °C to preserve activity across multiple experiments.