Anti-IKK alpha + beta (phospho S176 + S180) Antibody

This phospho-specific rabbit polyclonal antibody from Antibodies.com selectively detects the activated forms of IKK alpha and IKK beta only when serine residues 176 and 180 in their activation loops are phosphorylated. Those serine residues sit in the canonical kinase activation segment, and their phosphorylation is required for full enzymatic activity in the canonical NF-kB signaling cascade. The reagent is widely used to track pathway activation triggered by pro-inflammatory cytokines such as TNF-alpha and IL-1beta, by Toll-like receptor ligands, or by cellular stress conditions including genotoxic and oxidative challenge.

Supplied as 50 µl of IgG in an unconjugated format, the antibody can be paired with HRP-, fluorophore-, or biotin-labeled secondary reagents of the user's choice. Western blot is the validated primary application, and reactivity has been confirmed against human and mouse samples. Polyclonal rabbit host and IgG isotype help generate strong signal on whole-cell lysates prepared under standard denaturing conditions, while the unconjugated presentation preserves flexibility for custom labeling strategies.

Common research contexts include dissecting TNF receptor proximal signaling, profiling innate immune activation, characterizing NF-kB pathway involvement in cancer models and chronic inflammatory disease, and probing upstream kinase events that drive NF-kB-dependent gene expression. The unconjugated format also allows use in immunoprecipitation workflows aimed at recovering phosphorylated IKK complexes before downstream mass spectrometry identification or kinase activity assays.