Anti-IKK alpha + beta (phospho S176 + S180) Antibody

Raised in rabbit and supplied by Antibodies.com as an unconjugated IgG polyclonal, this phospho-specific reagent targets the activating phosphorylation of IKK alpha (IKKα) and IKK beta (IKKβ) at serine 176 and serine 180. These adjacent residues lie within the T-loop of the IκB kinase complex, and their modification is the molecular switch that engages canonical NF-κB signaling downstream of TNF receptor, IL-1 receptor, and TLR stimulation. The antibody is validated for western blot on human and mouse samples, allowing researchers to monitor stimulus-dependent IKK activation in cell lysates and tissue extracts without interference from the unmodified kinase pool. Each vial contains 200 µl of stock, with the polyclonal format providing tolerance for minor sequence variation between IKKα and IKKβ around the shared phospho-epitope, a useful feature when probing primary cells where pathway readouts can be transient and sub-stoichiometric.