Anti-IKK alpha + beta (phospho S176 + S180) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com is raised against the IKKα/IKKβ kinase complex, selectively recognizing the activated form phosphorylated at Ser176 of IKKα and the equivalent Ser180 site of IKKβ. Because these activation-loop residues are direct targets of upstream kinases such as TAK1 and MEKK3, the reagent serves as a sensitive read-out of canonical NF-κB pathway engagement rather than total IKK protein levels.

The antibody is validated for Western blot and is reactive with both human and mouse samples, enabling comparative signaling studies across species. Supplied as an unconjugated preparation at 100 µl, it can be paired with an HRP- or fluorophore-labeled secondary antibody of choice and is compatible with standard PVDF or nitrocellulose workflows. The polyclonal format gives broad coverage of the phospho-epitope, which helps when the activation status of multiple IKK species must be captured in a single lane.

Typical uses include monitoring stimulus-dependent IKK activation downstream of TNFα, IL-1, LPS, or PMA treatment, profiling pathway inhibition by candidate IKK or NF-κB inhibitors, and confirming knockout or knockdown phenotypes in cell lines and primary cells. For best stability, aliquot on receipt, store refrigerated, protect from light, and avoid repeated freeze-thaw cycles of the working stock.