Anti-IKK a (Ab-23) Antibody

Raised in rabbit against the N-terminal region of inhibitor of nuclear factor kappa-B kinase subunit alpha (IKKα), this polyclonal reagent from Antibodies.com is supplied as an unconjugated 100 µl liquid stock for western blot (WB) and immunohistochemistry (IHC) workflows.

IKKα, also known as CHUK or IKK1, operates as a serine/threonine kinase within the IKK complex, where it phosphorylates IκBα to drive canonical NF-κB pathway activation. It is broadly expressed across human, mouse, and rat tissues, where it controls inflammatory signaling, immune cell activation, and cellular stress responses. Researchers apply this antibody to whole-cell lysates, tissue homogenates, and fixed paraffin-embedded sections to monitor IKKα expression under basal and stimulus-induced conditions.

The polyclonal format provides epitope redundancy, which helps when probing denatured targets on membranes or fixed antigens in IHC. The 100 µl volume supports multiple replicate blots and staining runs, while the unconjugated format allows flexible pairing with secondary antibodies labeled with HRP, biotin, or fluorophores depending on the chosen detection chemistry. For storage, aliquot upon receipt and freeze at -20 °C to preserve activity across freeze-thaw cycles.

This product is intended for research use only and is suitable for laboratories investigating NF-κB pathway biology, cytokine signaling, innate immunity, and inflammation-related disease models.