Anti-IKKγ (phospho-S31) Antibody

This polyclonal antibody from Antibodies.com specifically detects IKKβ when phosphorylated at serine 31, an autophosphorylation site within the kinase domain. Raised in rabbit hosts, the reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2, packaged as 100 µl. It is validated for Western blot applications and shows reactivity with human, mouse, and rat samples, supporting studies of NF-κB signaling, inflammatory cascades, and kinase regulation across mammalian model systems.

The phospho-S31 epitope marks an autophosphorylation event that distinguishes activated IKKβ pools from total cellular IKKβ, allowing researchers to resolve activation dynamics independently of expression levels. Because the antibody is unconjugated, it pairs readily with HRP-, fluorescent-, or biotin-labeled secondary reagents chosen for a given detection platform. Sodium azide is included as a preservative; for downstream applications sensitive to azide, brief dialysis or buffer exchange is recommended before conjugation or live-cell work. Short-term storage at 4°C is acceptable for working aliquots, while long-term stability is best maintained by freezing at -20°C in single-use aliquots to limit freeze-thaw cycles.