Anti-IKKγ Antibody

Sourced from Antibodies.com, this primary antibody is a rabbit polyclonal raised against IKK-γ (also known as IKK-gamma/NEMO), the regulatory subunit of the IκB kinase complex central to NF-κB signaling. The unconjugated IgG reagent is validated for Western Blotting, Immunohistochemistry and Immunofluorescence workflows and shows broad cross-reactivity across human, mouse and rat samples, making it a flexible tool for studies in inflammation, cancer and signal transduction. It is supplied as a liquid formulation at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant, shipped in a 50 µl volume suitable for multiple experiments. Recommended working dilutions typically range from 1:500 to 1:1000 for Western Blot and 1:100 to 1:500 for Immunofluorescence, with optimal conditions to be determined by the end user. The antibody is affinity-purified from rabbit serum using an antigen-specific peptide derived from the human IKK-γ sequence, and no cross-reactivity has been reported with IKK-alpha or IKK-beta. For storage, the manufacturer advises keeping the vial at -20 °C in aliquots to avoid repeated freeze-thaw cycles, and the product is intended for research use only. With worldwide dispatch from the Antibodies.com logistics hubs and full technical support, this reagent is a reliable choice for laboratories probing NF-κB pathway activation.