Anti-IKKα/β (G182) Antibody

Raised in rabbit against an epitope mapping near amino acid G182, this affinity-purified polyclonal reagent from Antibodies.com is designed for the detection of IKKα and IKKβ, the catalytic subunits of the IκB kinase complex that governs NF-κB activation through phosphorylation at Ser176/Ser180 (IKKα) and Ser177/Ser181 (IKKβ). The unconjugated antibody is supplied at 1 mg/ml of total rabbit IgG in phosphate-buffered saline (pH 7.2) containing 0.02% sodium azide and 50% glycerol as a cryoprotectant, with the manufacturer's stated purity exceeding 95% by SDS-PAGE following affinity-chromatographic purification. The recommended application is immunohistochemistry on formalin-fixed, paraffin-embedded sections, used at a working dilution of 1:50 to 1:200, and validated reactivity is reported against human, mouse, and rat tissue (with endogenous IKKα/β detected at the expected molecular weight of approximately 85 kDa, corresponding to UniProt entries O15111 and O14920). The 50 µl vial is shipped in liquid format and should be stored short-term at 4 °C; for longer-term stability the supplier recommends aliquoting and freezing at -20 °C while avoiding repeated freeze-thaw cycles. As a research-use-only polyclonal reagent, it provides a convenient tool for characterising IKK signalling in the Antibodies.com catalogue of primary antibodies, complementing other IKKα/β clones (such as the Q174 and D173 versions) that target adjacent epitopes within the same conserved kinase domain.