Anti-IKBKB Antibody

A rabbit polyclonal antibody to IKBKB is offered by Antibodies.com as a primary antibody reagent. The antibody is unconjugated and of the IgG isotype, raised in rabbit. The catalogue entry lists reactivity with human and mouse samples and applications in Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF).

According to the manufacturer's data for the corresponding reagent, the antibody was generated against a synthetic peptide corresponding to amino acids 500–600 of human IKKβ (NCBI reference NP_001547.1) and purified by affinity chromatography. The product is supplied as a liquid in phosphate buffered saline at pH 7.3, containing 50% glycerol and 0.05% Proclin 300, with a lot-specific concentration. The target protein has a reported molecular weight of 87 kDa. The manufacturer's data additionally lists reactivity with rat samples and recommends dilutions of 1:500–1:1,000 for WB and 1:50–1:200 for IHC; the reagent is supported by four scientific validation images. The product is shipped at 4°C and, on receipt, should be aliquoted and stored at –20°C, with repeated freeze/thaw cycles avoided.