Anti-IkB-e (Ab-22) Antibody

Raised in rabbit and affinity-purified by epitope-specific peptide chromatography, this polyclonal reagent from Antibodies.com targets IκB-ε and is supplied at a robust 1.0 mg/mL in 50 µL aliquots. The immunogen is a synthetic peptide spanning residues 20–24 (I-E-S-L-R) of human IκB-ε, and the antibody specifically detects the endogenous total IκB-ε protein in validated Western blot (WB) and immunohistochemistry (IHC) workflows. It is unconjugated, allowing flexible downstream labelling, and has been verified to cross-react with Human, Mouse, and Rat samples, making it a versatile choice for comparative studies across these species. The buffer formulation is phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide as preservative, and 50% glycerol for cryoprotection. For optimal long-term preservation, the antibody should be stored at −20 °C, with short-term use permitted at 4 °C; it is shipped at 4 °C, and repeated freeze/thaw cycles should be avoided to maintain binding activity. As a polyclonal preparation, it offers broad epitope coverage of IκB-ε, an NF-κB inhibitory protein relevant to inflammatory signalling research, and is well-suited for laboratories investigating IκB family regulation in cell and tissue lysates.