Anti-IkB-α/NFKBIA Antibody

This rabbit polyclonal antibody from Antibodies.com targets IkB-α (also known as NFKBIA), a cytoplasmic inhibitor of the NF-κB transcription factor. IkB-α sequesters NF-κB dimers in resting cells by masking their nuclear localization signals; upon stimulation by cytokines or stress, IKK-mediated phosphorylation triggers ubiquitination and proteasomal degradation, freeing NF-κB to drive transcription of inflammatory, anti-apoptotic, and immune response genes.

Validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation, the reagent detects endogenous protein in human, mouse, and rat samples. The polyclonal format raises antibodies against multiple epitopes, increasing tolerance to minor denaturation and supporting robust signal across applications including IP pull-downs and IF imaging of NF-κB pathway activation.

Supplied as 100 µl at 1 mg/ml in PBS with 0.1% Sodium Azide and 50% Glycerol, the unconjugated preparation can be directly labeled or paired with a secondary antibody of the researcher's choice. Investigators studying inflammation, innate immunity, cancer signaling, or NF-κB pathway dysregulation will find this reagent useful for tracking endogenous IkB-α levels and phosphorylation dynamics.