Anti-IgG3 Antibody (HRP) [KT133]

The Antibodies.com reagent is a mouse monoclonal IgG1 antibody that specifically recognises human IgG3, directly conjugated to horseradish peroxidase (HRP) for streamlined ELISA detection. Each vial contains 100 µg of purified antibody in a ready-to-use liquid format, allowing direct addition to microwell plates without the need for a separate secondary antibody incubation. The antibody is widely used for IgG3 subclass quantification in hybridoma screening, isotype profiling of monoclonal antibody preparations, and characterisation of purified immunoglobulin fractions. Internal validation at Antibodies.com demonstrated strong reactivity with serum-derived and recombinant human IgG3, with no significant cross-reactivity against human IgG1, IgG2, or IgG4 subclasses. Recommended storage is at 4°C protected from light, with the working dilution freshly prepared from the stock to preserve HRP enzymatic activity across multiple freeze-thaw cycles.