Anti-IgG2b Antibody [KT93]

The Anti-IgG2b Antibody [KT93] from Antibodies.com is a rat-derived monoclonal reagent that specifically recognizes the mouse IgG2b immunoglobulin subclass. Supplied as a liquid preparation at 200 µg, this unconjugated primary antibody enables direct detection in immunoassays without the need for secondary labeling. The KT93 clone has been validated for ELISA and Western blot workflows, supporting quantitative and semi-quantitative analysis of mouse IgG2b in cell culture supernatants and tissue lysates. With its IgG1 isotype background, KT93 exhibits subclass-restricted binding, minimizing cross-reactivity with IgG1, IgG2a, and IgG3, which makes it a reliable reagent for isotype profiling in mouse immunological studies. Researchers commonly apply it at working concentrations of 1–5 µg/mL for ELISA coating and 1–2 µg/mL for Western blot detection. The reagent is intended for research use only and should be stored frozen at -20 °C in single-use aliquots to preserve activity across multiple freeze-thaw cycles.