Anti-IgG Antibody (HRP) [KT131]

The KT131 clone is a Mouse monoclonal antibody that specifically binds human IgG, conjugated to horseradish peroxidase (HRP) for direct chemiluminescent or colorimetric detection without needing a secondary antibody step. Provided as a liquid formulation at 100 µg per vial, the reagent is validated for ELISA, western blot, and sELISA workflows.

Working dilutions typically range from 1:1,000 to 1:10,000 in ELISA and 1:2,000 to 1:5,000 for western blot, with empirical optimization recommended for each assay. The conjugate is specific for human IgG, showing minimal cross-reactivity with mouse, rat, or rabbit immunoglobulin subclasses, which makes it a reliable tool for hybridoma screening, immunoglobulin quantitation, and monoclonal antibody characterization studies. Aliquot upon receipt and store at -20 °C, keeping working stocks at 4 °C and protected from light to preserve HRP enzymatic activity; avoid repeated freeze-thaw cycles. Supplied by Antibodies.com for research use only.