Anti-IGF1 Antibody

The Insulin-like Growth Factor 1 (IGF1) target is a 70 amino acid polypeptide hormone structurally related to proinsulin that signals through the IGF1 receptor (IGF1R) and governs cellular proliferation, differentiation, survival, and metabolic activity. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied at 1 mg/ml in Phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2, and is validated for Western blot detection of endogenous IGF1 in human and rat sample lysates.

Researchers studying growth axis signaling, endocrine regulation, and tumor biology rely on polyclonal anti-IGF1 antibodies to capture full-length and processed isoforms, including the mature 7.5 kDa peptide and higher-molecular-weight IGF1 binding protein complexes often observed on Western blots. The polyclonal format offers multi-epitope recognition of the IGF1 molecule, increasing the likelihood of detecting both the circulating mature hormone and precursor forms during immunoblotting of cell lysates and tissue homogenates.

Recommended handling: keep the 100 µl vial refrigerated and protect from light to preserve antibody activity across its working shelf life. Sodium azide at 15 mM is included as a preservative to prevent microbial growth; avoid freeze-thaw cycles that can denature the immunoglobulin fraction. For Western blot, dilute in accordance with your standard blocking buffer and probe PVDF or nitrocellulose membranes using established transfer conditions; downstream chemiluminescent detection reagents compatible with rabbit primary antibodies are appropriate. Suitable for research use only in applications spanning cancer cell signaling, metabolic disease models, and developmental biology studies.