Anti-IGF-1R (Ab-1346) Antibody

Raised in rabbits against a synthetic peptide spanning residues 1344–1348 (Q-P-Y-A-H) of human IGF-1R, this affinity-purified polyclonal IgG reagent from Antibodies.com binds the endogenous receptor around the activating tyrosine 1346 phosphorylation site, making it a useful probe for total IGF-1R levels in ligand-stimulation and kinase-inhibitor studies. The antibody is supplied at 1.0 mg/mL in a 100 µl vial of phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is unconjugated so users can pair it with their own HRP, biotin, or fluorophore-labeled secondary reagents.

Validated applications include Western blot on 293 cell extracts and immunohistochemistry on paraffin-embedded human breast carcinoma tissue, with cross-reactivity confirmed against Human, Mouse, and Rat samples. The predicted receptor mass of approximately 200 kDa (with a 95 kDa precursor band) supports clear band identification in standard SDS-PAGE workflows. Researchers deploying this reagent commonly characterize receptor expression in tumor sections, profile PI3K-AKT and RAS-MAPK pathway activation in cell line and xenograft models, and complement phospho-specific readouts with total-protein detection.

Aliquot upon first thaw and store at -20 °C for long-term use; the product is shipped refrigerated at 4 °C and is intended for research use only, not for diagnostic or therapeutic applications.