Anti-IFNAR1 Antibody

Raised in rabbits against a synthetic peptide of human IFNAR1 and affinity purified, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as 100 µl of liquid in Phosphate Buffered Saline (pH 7.30) with 0.02% sodium azide as a preservative. The antibody is validated for western blot, immunohistochemistry, and flow cytometry on human samples, with recommended starting dilutions of 1:500 to 1:1000 for western blot, 1:20 to 1:50 for IHC, and 1:20 to 1:50 for flow cytometry. The target band is observed near 70 kDa, consistent with the IFNAR1 subunit of the type I interferon receptor that drives JAK-STAT signaling downstream of IFN-α and IFN-β.

Because the reagent is unconjugated, it can be paired with HRP-conjugated secondary antibodies for chromogenic western blot, fluorophore-labeled secondaries for multicolor flow cytometry panels, or biotinylated secondaries for amplified IHC detection. The polyclonal format allows recognition of multiple epitopes on IFNAR1, which can improve signal in fixed tissue sections and across sample preparations where a single monoclonal epitope may be compromised by fixation or processing. Researchers should store the vial at -20°C in working aliquots to limit freeze-thaw cycles and preserve activity; thawed aliquots can be kept refrigerated for short-term use. The reagent is intended for research use only and is not validated for diagnostic or therapeutic applications.