Anti-IDH2 Antibody

The Anti-IDH2 Antibody from Antibodies.com is a rabbit polyclonal primary reagent raised against isocitrate dehydrogenase 2 (IDH2), the mitochondrial NADP-dependent enzyme that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate within the citric acid cycle. Supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2, the antibody is validated for Western blot (WB) applications and shows reactivity with human, mouse, and rat samples, supporting cross-species studies of cellular metabolism.

IDH2 is a focal target in cancer biology because somatic mutations in the gene, including the well-characterized R172 hotspot, confer neomorphic activity that produces the oncometabolite 2-hydroxyglutarate. Such mutations are recurrent in gliomas, acute myeloid leukemia, chondrosarcomas, and other solid tumors, driving widespread interest in tools that can resolve mitochondrial enzyme expression and regulation. The polyclonal format amplifies signal across multiple epitopes, which is useful when probing denatured IDH2 on membranes after SDS-PAGE and standard wet or semi-dry transfer.

Each vial contains 100 µl of antibody, a volume that supports numerous blotting experiments under typical loading conditions. The PBS-based formulation with sodium azide preserves reagent stability for routine benchtop storage and protects against microbial contamination during repeated use. Researchers studying mitochondrial bioenergetics, oxidative stress responses, tumor metabolism, or leukemia diagnostics can deploy this antibody in workflows that compare wild-type and mutant IDH2 expression across patient-derived samples, cell line panels, and engineered model systems.

As an unconjugated reagent, the antibody can be paired with HRP- or fluorophore-labeled secondary antibodies chosen to suit a given imaging or chemiluminescence detection platform, giving laboratories flexibility in experimental design. It is intended for research use only, in line with the broader Antibodies.com catalog of primary antibodies and supporting reagents.