Anti-ICAM1 Antibody [Assigned #A0307]

ICAM1 (also known as CD54) is a cell-surface glycoprotein of the immunoglobulin superfamily that mediates leukocyte adhesion and transendothelial migration during inflammatory responses. This unconjugated mouse monoclonal antibody, clone A0307 from Antibodies.com, was raised against human ICAM1 and is validated for Western blot and immunofluorescence applications on human samples.

Supplied as a 200 µL liquid aliquot in a vial, the reagent is a mouse IgG isotype antibody that recognizes the native human ICAM1 protein. Researchers commonly use it to probe ICAM1 expression in endothelial and immune cell models, where it supports detection of the glycoprotein across its mature, glycosylated molecular weight range. The unconjugated format gives full flexibility to pair the primary with a laboratory's preferred HRP- or fluorophore-conjugated secondary for either Western blot or immunofluorescence detection.

For long-term storage, keep the antibody frozen at -20°C in working aliquots to limit freeze-thaw cycles, and protect from light to preserve IgG integrity. A brief centrifugation before opening recovers the full 200 µL volume from the vial. Clone A0307 is one of the characterized Antibodies.com reagents for studying ICAM1 in human systems and is intended for research use only.