Anti-IARS2 Antibody

This polyclonal antibody reagent from Antibodies.com is raised in rabbit and targets IARS2 (isoleucyl-tRNA synthetase 2, mitochondrial), a nuclear-encoded mitochondrial enzyme that charges mitochondrial tRNA with isoleucine during organellar protein synthesis. The unconjugated IgG fraction is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, at approximately pH 7.3, and ships as a 100 µl vial suited to multiple western blot experiments.

Validated for WB detection across human, rat, and mouse samples, this reagent supports comparative mitochondrial biology research, including investigations of IARS2 mutations linked to CAGSSS syndrome, Leigh syndrome, and nonsyndromic hearing loss, as well as broader studies of mitochondrial aminoacyl-tRNA synthetase activity and translation fidelity. Storage recommendations include refrigeration at 4 °C, protection from light, aliquoting on first use, and avoiding repeated freeze-thaw cycles to preserve antibody activity over the shelf life.

Recommended western blot dilutions generally fall between 1:500 and 1:2000 depending on target abundance, and the antibody is intended for research use only. Investigators studying mitochondrial translation, OXPHOS assembly, or tissue-specific IARS2 knockout models will find this polyclonal reagent a useful tool for expression profiling and immunoblotting of mitochondrial extracts.