Anti-HXK I Antibody

Manufactured by Bioworld Technology and distributed through Antibodies.com under catalog number A32378, this recombinant rabbit monoclonal reagent is raised against hexokinase I (HXK I) and is supplied as a 50 µl aliquot of unconjugated antibody. The product is formatted in 1×TBS (pH 7.4) supplemented with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, and the IgG is Protein A affinity purified for a clean, consistent preparation. Endogenous levels of HXK I — the approximately 102 kDa "brain-form" hexokinase — are detected without cross-reactivity to related family members, making the clone a reliable tool for glucose-metabolism studies. Validation on the Antibodies.com product page covers a broad application panel that includes Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry (IHC), and flow cytometry (FC), so the same lot can be used across imaging, lysate, and cell-staining workflows. Reactivity has been confirmed in human, mouse, and rat samples, allowing cross-species experimental design from a single reagent. The unconjugated format gives users full flexibility to pair the antibody with their preferred secondary — for example goat anti-rabbit IgG labeled with HRP, AP, biotin, or FITC — depending on the detection chemistry required. For storage, the recommended conditions consistent with this formulation are short-term holding at 4 °C and longer-term aliquoting at −20 °C to preserve activity across many experiments.