Anti-HXK I Antibody

A recombinant rabbit monoclonal reagent that selectively binds Hexokinase I, the enzyme catalyzing the first committed step of glycolysis by phosphorylating glucose to glucose-6-phosphate. Distributed by Antibodies.com as a 100 µl aliquot in 1×TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, this unconjugated antibody is validated across western blot, immunocytochemistry and immunofluorescence, immunohistochemistry, and flow cytometry workflows. Cross-reactivity has been confirmed for human, mouse, and rat samples, supporting translational studies that compare HK I expression across model organisms. Researchers commonly deploy this reagent to investigate Warburg-effect biology in tumor cell lines, to map hexokinase distribution in neural and pancreatic tissue sections, and to probe mitochondrial outer-membrane binding of HK I under metabolic stress. Because the antibody is recombinant and monoclonal, lot-to-lot consistency is preserved across production runs, and the unconjugated format leaves the choice of fluorophore, biotin, or HRP label to the end user. Short-term storage at 4 °C is appropriate for working aliquots, while the glycerol-containing buffer maintains long-term stability at -20 °C.