Anti-HTR1A Antibody

Raised in rabbit against a synthetic peptide from the internal region of human HTR1A, this unconjugated polyclonal IgG from Antibodies.com recognizes endogenous levels of the 5-hydroxytryptamine receptor 1A, a G protein-coupled receptor (UniProt P08908) that serves as both a somatodendritic autoreceptor on serotonergic raphe neurons and a postsynaptic heteroreceptor on hippocampal and cortical targets. Validated for western blot on human, mouse, and rat samples, the reagent is supplied as a 100 µl liquid aliquot in PBS with 50% glycerol, ready to pair with HRP- or fluorophore-conjugated secondaries for chemiluminescent or fluorescent detection.

Predicted molecular weight of the receptor is approximately 46 kDa, with western blot bands typically observed near 40 kDa depending on glycosylation state. The polyclonal pool binds multiple epitopes across the receptor, useful for probing low-abundance expression or modified receptor variants in studies of antidepressant mechanism, 5-HT1A biased agonism (e.g. NLX-101), and rs6295 promoter polymorphism effects on autoreceptor versus heteroreceptor expression.

Researchers mapping receptor localization in hippocampus, dorsal raphe nuclei, and cortex, or characterizing 5HT1AR-FGFR1 heteroreceptor complexes in genetic rat depression models, can combine this antibody with transcript-level and ligand-binding assays. Shipped refrigerated; aliquot upon arrival and store at -20°C between uses to preserve binding activity across repeated experiments. For research use only.