Anti-HSPB1 (phospho S82) Antibody - Identical to Abcam (ab210810)

Manufactured by Antibodies.com as a primary antibody, this rabbit polyclonal reagent recognises HSPB1 (also known as Hsp27) when phosphorylated at serine 82. The unconjugated IgG antibody is raised against a synthetic phosphorylated peptide surrounding S82 of human HSP27/HSPB1 (NP_001531.1).

Validated applications include Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP), with immunofluorescence (IF) additionally listed as a tested application in the Antibodies.com product entry. Recommended working dilutions are WB 1:500–1:2,000, IHC 1:50–1:200, and IP 1:50–1:100. The reagent is reported to react with human samples; rat reactivity is also noted in the Antibodies.com catalogue listing.

The antibody is supplied as a liquid in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.01% Thiomersal, and is purified by affinity chromatography. The target protein is reported at a molecular weight of 28 kDa. The product is shipped at 4°C and, upon delivery, should be aliquoted and stored at -20°C, with freeze/thaw cycles to be avoided.