Anti-HSPB1 (phospho S82) Antibody

Phosphorylation of HSPB1 (also known as HSP27) at serine 82 marks activation of the p38 MAPK / MAPKAPK2/3 stress-response cascade, and tracking this modification is a common readout in cancer biology, neurobiology, and cell signalling research. This polyclonal antibody from Antibodies.com is raised in rabbit and selectively recognizes the phospho-S82 form of HSPB1, allowing researchers to distinguish the activated, phosphorylated pool from the basal, unphosphorylated chaperone pool in their lysates and tissue sections.

The reagent is supplied as an unconjugated IgG in a 200 µl volume, ready for direct labelling or for pairing with a chosen secondary detection reagent. It is reactive with human samples and has been validated by the manufacturer for Western blot (WB) and immunohistochemistry (IHC) workflows. For Western blot, common applications include probing cleared lysates from heat-shocked, oxidatively stressed, or cytokine-treated cultures to follow the kinetics of HSPB1 phosphorylation over time; for IHC, the antibody is used on formalin-fixed paraffin-embedded tissue sections to localize phospho-HSPB1 in tumour and stress-responsive compartments.

Provided by Antibodies.com as part of their primary antibody portfolio, this polyclonal reagent offers the flexibility of an unconjugated format, allowing researchers to pair it with HRP, fluorescent, or biotin-based secondaries according to their experimental design.