Anti-HSPB1 (phospho S15) Antibody

This polyclonal antibody from Antibodies.com targets HSPB1 (Heat Shock Protein Beta-1, also known as HSP27) when phosphorylated at serine 15. The S15 phospho-site is installed by MK2/MAPKAPK2 downstream of p38 MAPK and is widely used as a read-out of cellular stress pathway activation, cytoskeletal remodeling, and survival signaling under thermal, oxidative, or inflammatory challenge. The reagent is raised in rabbit, belongs to the IgG isotype, is supplied unconjugated in a 50 µl vial, and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows.

Reactive with human HSPB1, it supports research into cancer cell signaling, neurodegeneration, cardiovascular stress responses, and other models where p38-mediated HSP27 phosphorylation is being tracked. Because the reagent is polyclonal, it recognizes multiple epitopes around the phospho-S15 site, which can be advantageous when monitoring dynamic changes in HSPB1 phosphorylation status following stimuli such as heat shock, oxidative stress, or cytokine exposure. The unconjugated format allows users to pair it with their own secondary antibody or label of choice for fluorescence or chromogenic detection.

For handling, store the vial refrigerated, protect from light, and aliquot upon first use to limit freeze-thaw cycles of the antibody solution.