Anti-HSPB1 (phospho S15) Antibody

This polyclonal antibody from Antibodies.com targets HSPB1 (also known as HSP27) when phosphorylated at serine 15, a post-translational modification linked to activation of the p38 MAPK stress-response cascade. Raised in rabbit and supplied as an IgG polyclonal at 200 µl per vial, the reagent is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human samples.

Researchers studying heat shock protein signaling, actin cytoskeleton remodeling, and cellular stress responses use this antibody to detect the early phosphorylation event at S15, which precedes and triggers subsequent phosphorylation at sites such as S78 and S82. Common applications include profiling cancer cell lines in which HSPB1 phosphorylation is dysregulated, mapping stress signaling in ischemia-reperfusion and cardiac injury models, and characterizing neurodegenerative tissue samples. Unconjugated formulation allows flexibility with secondary antibody choice and supports custom labeling protocols for multiplex imaging, including co-localization studies with cytoskeletal markers.