Anti-HSPB1 (phospho S15) Antibody

This phospho-specific polyclonal reagent from Antibodies.com selectively recognizes human HSPB1 (also known as HSP27) only when the protein is phosphorylated at serine 15, a modification catalyzed by MAPKAP kinase 2 downstream of p38 MAPK signaling. Raised in rabbit and supplied as an unconjugated IgG, the antibody is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human specimens.

Phosphorylation at S15 is a transient post-translational event triggered by cellular stress, including heat shock, oxidative challenge, and cytokine exposure, making this reagent useful for studies of stress granule formation, cytoskeletal remodeling, and apoptotic regulation. The 100 µL volume provides enough material for multiple blots and staining experiments. Researchers investigating cancer cell survival, neurodegeneration, and cardiac ischemia commonly use phospho-S15 detection to monitor HSP27 activation dynamics in both fixed tissue sections and cell lysates.

For storage, aliquot the antibody upon arrival to limit freeze-thaw cycles and preserve phospho-specific binding activity over time. Each preparation is quality controlled for selective recognition of the phosphorylated epitope through peptide competition analysis, ensuring that signal in western blot and immunofluorescence reflects the modified form of HSPB1 rather than total protein levels.