Anti-HSPB1 Antibody

HSPB1 (also known as HSP27) is a 27 kDa small heat shock protein that functions as a molecular chaperone and participates in stress response, actin cytoskeleton organization, and inhibition of caspase-mediated apoptosis. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against HSPB1 and validated for western blot and immunofluorescence workflows on human, mouse, and rat samples.

Each vial is supplied as 100 µl of affinity-purified antibody in a buffer suitable for direct use in standard immunodetection protocols. The polyclonal format offers recognition of multiple epitopes across the HSPB1 sequence, which can be advantageous when probing phosphorylated or post-translationally modified forms of the protein alongside total HSPB1 expression.

Typical research contexts include studies of heat shock and oxidative stress signaling, cancer biology (HSPB1 overexpression has been linked to tumor progression and chemoresistance in breast, ovarian, and gastric carcinomas), neurodegeneration, and cardiovascular injury models. The unconjugated format lets researchers pair the antibody with a secondary label of choice, including HRP, Alexa Fluor, or biotin conjugates, depending on the downstream application.

Store refrigerated in the dark to preserve antibody activity, avoid repeated freeze-thaw cycles, and aliquot upon first use. For research use only.