Anti-HSP90B (Ab-254) Antibody

Produced in rabbit and supplied as a 100 µl aliquot in a vial, this unconjugated polyclonal antibody from Antibodies.com recognizes HSP90B1, the endoplasmic reticulum chaperone also known as gp96, GRP94, or endoplasmin. Validated for western blot, immunohistochemistry, and immunofluorescence on human samples, it suits studies of ER-resident chaperone biology and protein quality control.

HSP90B1 is the most abundant glycoprotein in the ER lumen, where it assists folding of client proteins such as integrins, toll-like receptors, and immunoglobulins, and contributes to calcium homeostasis via calnexin and calreticulin. Because expression rises sharply under ER stress and in many tumor types, the antibody is widely used as a marker of unfolded protein response activation and cancer cell stress biology.

The polyclonal format means the reagent recognizes multiple epitopes on HSP90B1, which can improve detection across western blot and immunofluorescence preparations. The unconjugated format allows researchers to pair it with HRP-, fluorophore-, or biotin-conjugated secondary antibodies matched to rabbit IgG, and it fits standard chromogenic IHC protocols on formalin-fixed paraffin-embedded tissue. For long-term storage, aliquot the reagent and keep frozen to limit freeze-thaw cycles; for short-term bench handling, refrigerate and protect from light.