Anti-HSP70 Antibody

HSP70 proteins are ATP-dependent molecular chaperones that guide nascent polypeptide folding, block aggregation of misfolded clients, and refold stress-denatured substrates during heat shock, oxidative challenge, and other proteotoxic insults. This unconjugated monoclonal reagent from Antibodies.com binds the inducible ~70 kDa HSP70 isoform and ships as 100 µl of purified mouse IgG.

The antibody has been validated for Western blot and immunofluorescence, where it detects endogenous HSP70 in lysates and fixed cells from human, mouse, and rat samples. Investigators apply it to track stress-induced HSP70 induction in tumor cell lines, probe chaperone responses in neurodegeneration and ischemia models, and compare cytosolic versus nuclear HSP70 distribution before and after heat shock. Monoclonal format ensures reproducible band patterns across repeat blots and staining sessions.

For short-term use keep refrigerated; aliquot and freeze at -20°C for long-term storage to limit loss of titer from repeated freeze-thaw cycles. On Western blot, dilute according to target abundance and reveal with an HRP- or fluorophore-conjugated anti-mouse IgG secondary. For immunofluorescence, pair with appropriate fixation and permeabilization to access intracellular HSP70 pools.