Anti-HSP27 (phospho-S82) Antibody

The HSP27 phospho-S82 polyclonal reagent from Antibodies.com is a rabbit-host primary antibody raised against the phosphorylated Ser82 residue of HSP27 (also known as HSPB1), a small heat shock protein whose modification is driven by the p38 MAPK / MAPKAP kinase 2 cascade during cellular stress. By recognizing the S82 phospho-epitope specifically, the antibody allows researchers to follow dynamic changes in HSP27 phosphorylation that accompany heat shock, oxidative stress, cytokine signaling, and growth factor stimulation, rather than measuring total HSP27 protein levels.

Validated for Western blot and immunohistochemistry, the reagent performs across human, mouse, and rat samples, supporting comparative studies in cancer biology, models of neurodegeneration, and investigations of the stress response where altered HSP27 phosphorylation has been documented. Because S82 phosphorylation governs HSP27 oligomerization and its chaperone function, this phospho-specific reagent is useful for work on actin cytoskeleton remodeling, apoptosis regulation, and cytoprotective mechanisms in a range of primary cells and tissue sections.

The antibody is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2, in a 100 µl volume sufficient for multiple blots and staining runs. The unconjugated format offers flexibility to pair with the researcher's preferred secondary detection reagents, and storage at 4 degrees C in the supplied buffer helps preserve phospho-epitope recognition across repeated use.