Anti-Hsp27 Antibody

Heat shock protein 27 (Hsp27), encoded by the HSPB1 gene, is a stress-responsive molecular chaperone that protects cells from heat shock, oxidative damage, and apoptotic triggers by stabilizing partially folded proteins and modulating actin cytoskeleton dynamics. This unconjugated mouse monoclonal IgG1 reagent from Antibodies.com targets Hsp27 for Western blot and immunocytochemistry workflows, with confirmed reactivity against human and monkey samples. The 100 µl format provides ample volume for multiple blotting membranes or coverslip preparations, and the unconjugated format allows researchers to pair the antibody with their preferred secondary detection system or custom labeling chemistry.

Phosphorylated Hsp27 (Ser78, Ser82) drives a shift between small oligomers and large chaperone-active aggregates, a transition that has been investigated in cancer cell survival, cardiac preconditioning, and motor neuron disease models. Researchers studying stress granule assembly, proteostasis in aging neurons, or chemoresistance mechanisms in breast and prostate carcinoma have used anti-Hsp27 antibodies to track expression changes and subcellular relocalization following proteotoxic insult. Aliquoting upon arrival and storing at 4 °C for short-term use, or freezing single-use aliquots at -20 °C for long-term stability, is the standard handling approach for this reagent.