Anti-HSP27 Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against heat shock protein 27 (HSP27) and supplied at a concentration of 1 mg/ml in a 50 µl liquid volume formulated in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The immunogen is a synthesized peptide derived from human HSP27 around the non-phosphorylation site of serine 78, and the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen, producing an unconjugated primary antibody that specifically detects endogenous levels of HSP27 protein. Validated applications include Western blotting (WB), immunohistochemistry (IHC) and ELISA, with confirmed reactivity against human samples. Recommended handling is storage at -20 °C for up to one year, after which antibody performance should be re-evaluated. Intended strictly for research use, this HSP27 polyclonal is well suited to laboratories studying heat shock chaperone biology, cellular stress responses, and the phosphorylation-dependent regulation of HSP27 in signalling and disease models.