Anti-HSP27 Antibody

Manufactured by Antibodies.com and distributed through AFMS, this Mouse Monoclonal primary antibody targets Heat Shock Protein 27 (HSP27), also known as HSPB1, and is supplied as a 50 µL liquid aliquot of purified immunoglobulin at a working concentration suitable for routine immunodetection. The reagent is unconjugated and arrives in a Tris-Glycine buffer formulation containing 0.1 M Tris-Glycine at pH 7.4, 150 mM NaCl, 0.2% sodium azide as a preservative, and 50% glycerol for stability. The host species is Mouse and the antibody is monoclonal, with an IgG1 isotype reported for the equivalent HSP27 G3.1 clone widely cited for this target. Verified applications on the Antibodies.com catalog page are Western Blot (WB) and Immunocytochemistry (ICC), with a recommended WB starting dilution of 1:100 and an observed molecular weight around 27 kDa, consistent with the predicted 23 kDa HSPB1 polypeptide on Swiss-Prot entry P04792. Reactivity has been confirmed on Human and Monkey samples, supporting studies in MCF-7 and other primate cell models commonly used to examine heat shock responses, cytoskeletal regulation, and estrogen-related signalling. For storage, the supplier recommends keeping the vial at 4 °C for short-term use, then aliquoting and transferring to -20 °C for long-term storage, with freeze-thaw cycles to be avoided. The product is supplied at 0.1 mg/mL, validated specifically for endogenous HSP27 detection without significant cross-reactivity to HSP70, HSP90, or related heat shock proteins, making it well suited for stress response, chaperone, and biomarker research workflows in academic and industrial laboratories.