Anti-HSP27 Antibody

This rabbit polyclonal primary antibody from Antibodies.com targets HSP27 (also known as HSPB1), a 27 kDa small heat shock protein that functions as an ATP-independent molecular chaperone. Raised in rabbit and supplied unconjugated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, the reagent is provided as a 100 µl aliquot in a single tube and is validated for western blot and immunofluorescence workflows across human, mouse, and rat samples.

The antibody detects endogenous HSP27 in cell lysates and cultured cells, supporting studies of cellular stress responses, cytoskeletal regulation, and apoptosis signaling, where HSPB1 phosphorylation at serine residues modulates oligomerization and client-protein binding. Because the preparation contains sodium azide, it should be handled with standard laboratory precautions and stored refrigerated for short-term use or frozen for longer-term stability, avoiding repeated freeze-thaw cycles to preserve reagent activity.