Anti-HSP27 (Ab-15) Antibody

Raised in rabbit, this polyclonal reagent targets human HSP27 (also designated HSPB1), a ~27 kDa small heat-shock protein that functions as an ATP-independent chaperone and is dynamically phosphorylated downstream of p38/MAPKAPK2 stress signaling. The 100 µl aliquot is unconjugated for flexible pairing with secondary reporters such as HRP, biotin, or fluorophores, and is validated on human samples in Western blot and immunohistochemistry workflows. Common uses include profiling heat-shock responses in tumor biopsies, tracking cytoskeletal remodeling under oxidative or cytokine challenge, and monitoring chaperone activation in cell lysates. Researchers comparing stress-kinase readouts often run this antibody alongside phospho-site-specific reagents to map HSP27 modification status between treated and untreated samples, and the unconjugated format supports custom detection strategies on fixed sections or transfer membranes. The reagent from Antibodies.com is supplied with lot-specific validation data and is intended for research use only.