Anti-HSF2 Antibody

Heat Shock Factor 2 (HSF2) is a stress-responsive transcription factor that binds heat shock elements in promoter regions and contributes to heat shock protein expression, spermatogenesis, and developmental signaling pathways. This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against HSF2 and recognizes an epitope conserved across human, mouse, and rat orthologs, enabling cross-species experimental work in cell and tissue models.

The antibody is supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol added as a cryoprotectant. For storage, aliquot the reagent into single-use portions upon arrival and store frozen at -20°C; this approach limits freeze-thaw cycles and helps maintain binding activity over multiple experiments. Protect from light during handling.

Validated applications include Western blot for detection of endogenous HSF2 protein levels in whole-cell or tissue lysates and immunofluorescence for subcellular visualization in cultured cells and fixed sections. The polyclonal format provides epitope redundancy, which can be useful when probing for isoforms, splice variants, or post-translationally modified forms of HSF2 in stress-response studies.