Anti-HSF1 (S303) Antibody

A rabbit polyclonal reagent targeting Heat Shock Factor 1 phosphorylated at Serine 303, this Antibodies.com antibody detects a regulatory phosphorylation mark involved in HSF1 transcriptional control during cellular stress responses. Serine 303 is a documented substrate of ERK and CK2 kinases, and its phosphorylation state is commonly examined in studies of proteostasis, chaperone regulation, and stress-kinase signaling pathways.

Each vial supplies 100 µl of unconjugated antibody at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2. The liquid format reduces freeze-thaw stress and simplifies aliquot handling for long-term storage, while the unconjugated presentation allows pairing with HRP, fluorescent, or biotinylated secondary reagents to suit individual Western blot workflows.

Validated for Western blot applications and reactive against human, mouse, and rat samples, the reagent supports phospho-specific detection of HSF1 in cell lysates and tissue extracts. Researchers commonly run the antibody alongside a total HSF1 loading control to distinguish changes in S303 phosphorylation from shifts in overall HSF1 protein abundance.