Anti-HSF1 (phospho S307) Antibody

This rabbit polyclonal IgG reagent from Antibodies.com specifically recognizes Heat Shock Factor 1 (HSF1) when phosphorylated at serine 307, a modification linked to negative feedback control of the heat shock transcriptional program. Supplied unconjugated at 200 µl per vial, the antibody is validated for Western Blot (WB) and Immunofluorescence (IF) workflows on human samples. Because phosphorylation at S307 modulates HSF1 DNA-binding activity and nuclear localization, detection of this phosphoepitope is informative in studies of acute heat stress, proteotoxic challenge, and recovery kinetics. The polyclonal format broadens recognition of the phospho-S307 neighbourhood, which helps when probing cell lysates from stressed cultures where the modification is transient or sub-stoichiometric. Unconjugated delivery leaves the detection chemistry open: HRP, Alexa Fluor, or biotin reporters can be paired to match a chosen imaging or blotting platform. Aliquot upon receipt to limit freeze-thaw cycles, store refrigerated for short-term use, and keep working stocks frozen for long-term stability. Intended for research use only, the reagent supports investigations into chaperone networks, stress-granule assembly, and HSF1-linked disease models in oncology and neurodegeneration.