Anti-HSF1 (phospho S307) Antibody

Phosphorylation at serine 307 modulates HSF1 transcriptional activity in response to cellular stress, and this unconjugated rabbit polyclonal reagent from Antibodies.com detects that phosphorylated epitope on human HSF1. Raised in rabbit and purified as an IgG preparation, the antibody is supplied as 100 µL of liquid and is validated for Western blot and immunofluorescence workflows on human samples.

Researchers studying heat shock response, proteostasis, or stress signaling use this reagent to probe HSF1 activation status in cell lysates and fixed cells. The polyclonal format provides broader epitope coverage than monoclonal alternatives, which is useful when monitoring dynamic phosphorylation changes. Because S307 phosphorylation reflects a regulatory node distinct from the more commonly studied S326 site, this reagent is often paired with total HSF1 blots to dissect pathway kinetics.

For storage, keep refrigerated and protect from light; aliquot upon receipt to limit freeze-thaw cycles of the original vial, and brief equilibration to room temperature before use is recommended for accurate pipetting.