Anti-HSF1 (Ab-307) Antibody

Manufactured by Antibodies.com and supplied through AFMS, this rabbit polyclonal reagent is raised against heat shock factor 1 (HSF1) and targets the region surrounding residue 307 of the human protein. The immunogen corresponds to the peptide sequence around amino acids 305–309 (P-Q-S-P-R) of human HSF1, and the antibody is affinity-purified by epitope-specific peptide chromatography after immunization with synthetic peptide-KLH conjugates. It specifically recognizes the endogenous level of total HSF1 protein in human samples and is validated for Western blotting (WB) and immunohistochemistry (IHC). The reagent is supplied as an unconjugated, affinity-purified polyclonal antibody at 1.0 mg/mL, in 50 µL and 100 µL pack sizes, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a stabilizer. The host species is rabbit, the clonality is polyclonal, the conjugate is unconjugated, and the reactivity profile is Human. According to the Antibodies.com specification sheet, the product is shipped at 4 °C; upon arrival, aliquots should be stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. The reagent is intended for research use only and is not for diagnostic or therapeutic applications. Researchers working on the heat-shock response, transcriptional regulation of stress genes, and HSF1-driven chaperone expression in human cell and tissue systems will find this antibody suitable for detecting total HSF1 levels under both basal and stress-induced conditions.