Anti-HSD3B2 Antibody

Offered through Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against HSD3B2, designed for the detection of endogenous HSD3B protein levels in research samples. The antibody was generated by immunizing rabbits with recombinant full-length human HSD3B, and the resulting antiserum was purified by affinity chromatography using an epitope-specific immunogen to a final purity of greater than 95% as judged by SDS-PAGE. The product is supplied at a working concentration of approximately 1 mg/mL in PBS containing 0.1% sodium azide and 50% glycerol, and is provided as a liquid in a 50 µL vial suitable for routine bench use. Recommended applications include Western blotting (WB) and immunohistochemistry (IHC), and the reagent has been validated for reactivity with both human and mouse samples, giving an expected band at approximately 42 kDa on Western blots. The antibody is unconjugated and of the rabbit IgG isotype, making it compatible with standard secondary antibody detection systems such as HRP- or fluorophore-conjugated anti-rabbit IgG. For long-term storage the vial should be kept at -20 °C, with short-term working aliquots stable at 4 °C for up to one month; repeated freeze-thaw cycles should be avoided to preserve activity. Intended for research use only, this Antibodies.com polyclonal reagent provides a reliable tool for laboratories investigating steroidogenic enzyme biology, androgen and progesterone biosynthesis pathways, and adrenal or gonadal tissue expression patterns of 3β-hydroxysteroid dehydrogenase isoforms.