Anti-HSD3B2 Antibody

Hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase 2 (HSD3B2) is an enzyme anchored in the endoplasmic reticulum and mitochondrial membranes of steroidogenic tissues, where it catalyzes the conversion of pregnenolone to progesterone and related steroid intermediates in the adrenal cortex and gonads. The protein is a focal point in studies of steroidogenesis, reproductive endocrinology, and adrenal pathophysiology, and loss-of-function mutations are a recognized cause of classical congenital adrenal hyperplasia. Researchers use this reagent to map enzyme expression across adrenal zonae, Leydig cells, theca interna, and placental trophoblast.

Supplied by Antibodies.com as 100 µl of unconjugated rabbit polyclonal antiserum at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the antibody is validated for Western blot on human, mouse, and rat samples. The expected band on Western blot is approximately 42 kDa, consistent with full-length HSD3B2. Aliquot upon receipt and store at -20 °C, avoiding repeated freeze-thaw cycles and protecting the vial from light.