Anti-HSD3B1 Antibody

Antibodies.com offers this rabbit polyclonal antibody raised against human 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1), a membrane-bound enzyme anchored in the endoplasmic reticulum that catalyzes key steps in steroid hormone biosynthesis, including conversion of pregnenolone to progesterone and DHEA to androstenedione. Supplied as 100 µl of unconjugated antiserum, the reagent is validated for Western blot (WB) and immunohistochemistry (IHC) on human tissue.

HSD3B1 expression is of strong interest in adrenal cortex, placenta, ovary, testis, and prostate tissue, where the enzyme influences glucocorticoid, mineralocorticoid, and sex steroid output. It has gained particular attention in prostate cancer research tied to the HSD3B1(367N) variant and the development of castration-resistant disease, making the antibody a useful tool for translational workflows that examine enzyme expression in tumor specimens and patient-derived models. It can also be applied to studies of steroidogenic pathways, adrenal hyperplasia, polycystic ovary syndrome, and other hormone-driven conditions.

For best results, store refrigerated, protect from light, and aliquot upon first use to minimize freeze-thaw cycles; this preserves polyclonal reactivity across repeated WB and IHC runs. The rabbit host offers recognition of multiple epitopes across the HSD3B1 protein, supporting detection in samples where expression may vary between tissues or disease states.