Anti-HSD17B13 Antibody

HSD17B13 (17β-hydroxysteroid dehydrogenase type 13) is a lipid droplet-associated enzyme expressed predominantly in hepatocytes, where it has emerged as a significant target in studies of non-alcoholic fatty liver disease and chronic liver injury. This rabbit polyclonal antibody from Antibodies.com recognizes the human HSD17B13 protein and is supplied as an unconjugated, 100 µl volume suitable for western blot and immunohistochemistry workflows.

The antibody is raised in rabbit and validated for detection of the native human target in both denaturing gel and fixed-tissue contexts. Researchers using this reagent commonly investigate HSD17B13 expression in liver biopsy sections and in lysates of hepatic cell lines, where altered enzymatic activity has been linked to lipid metabolism, inflammatory signaling, and fibrotic progression.

This unconjugated polyclonal reagent allows flexible selection of detection chemistries, including HRP, biotin, or fluorescent secondaries depending on the assay configuration. For western blot, typical protocols resolve HSD17B13 near its expected molecular weight and reveal expression differences between diseased and matched normal hepatic samples. For immunohistochemistry, the antibody supports chromogenic or immunofluorescent visualization of HSD17B13 distribution within hepatocyte cytoplasm and along lipid droplet membranes.

Storage at 4°C in the supplied vial is appropriate for routine short-term use; aliquoting is recommended to limit repeated freeze-thaw cycles if the reagent is reserved for long-term projects. Researchers are encouraged to titrate the antibody against their specific tissue or lysate preparation to determine optimal working dilutions for their chosen detection system.