Anti-HSD17B12 Antibody

HSD17B12 (hydroxysteroid 17-beta dehydrogenase type 12) is a membrane-associated enzyme that participates in the long-chain fatty acid elongation cycle and contributes to steroid hormone metabolism, making it a target of interest in metabolic and oncology research. This rabbit polyclonal reagent from Antibodies.com is raised against HSD17B12 and supplied as 100 µl of unconjugated antiserum suitable for Western blot (WB) and immunohistochemistry (IHC) workflows on human samples. The antibody detects the endogenous protein in tissue sections and immunoblots, supporting studies of lipid biosynthesis pathways, oestrogen-related signalling, and tumour biology where altered HSD17B12 expression has been reported. Because the target is a multi-pass endoplasmic reticulum enzyme, the reagent is best applied to membrane-enriched fractions or fixed cell preparations; researchers should optimise fixation and detergent conditions for IHC versus WB to preserve epitopes on the hydrophobic regions. The polyclonal format provides recognition of multiple epitopes on HSD17B12, which can be helpful when working with denatured samples in WB and with sections in IHC. For longer-term storage, aliquot and freeze to limit freeze-thaw cycles and preserve antibody activity.