Anti-HSD17B10 Antibody

HSD17B10 (17β-hydroxysteroid dehydrogenase type 10), also referred to as ABAD or ERAB, is a mitochondrial enzyme involved in fatty acid beta-oxidation, branched-chain amino acid catabolism, and the metabolism of steroid substrates; it has also been studied for its binding to amyloid-beta in Alzheimer's disease research. This polyclonal reagent from Antibodies.com is raised in rabbit and supplied as 100 uL of unconjugated antiserum, recognizing an epitope conserved across human, mouse, and rat samples. Confirmed applications include western blot, immunohistochemistry on fixed tissue sections, and immunofluorescence, supporting protein detection in cell culture, primary tissue, and subcellular mitochondrial fractions. For western blot, loading 10-20 ug of total protein is generally sufficient; transfer to PVDF or nitrocellulose and probing with the unconjugated antibody permits downstream chemiluminescent readout. The product should be stored at -20 degrees C in working aliquots to limit freeze-thaw cycles, with the stock vial kept frozen between uses to preserve activity. Each batch is affinity-purified and intended for research use only.